What do you want to know about the test kit?

https://www.tsktbiotech.com/pcr-qpcr-kits.html

Abstract

Background:

Malaria is a life-threatening parasitic disease transmitted through the bite of an infected female Anopheles mosquito. Light microscopy remains the traditional gold standard for malaria diagnosis. This study aimed to assess the diagnostic accuracy of two commercially available quantitative PCR (qPCR) kits compared to light microscopy and nested multiplex PCR (NM-PCR).

Methods:

A cross-sectional study was conducted in southwest Saudi Arabia involving 92 febrile patients who met the inclusion criteria. Plasmodium species were detected using light microscopy, NM-PCR, and two qPCR kits (RealStar and Viasure). Diagnostic parameters—sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and receiver operating characteristic (ROC) curves—were evaluated. Data were analyzed using SPSS version 25, with statistical significance set at p ≤ 0.05.

Results:

Light microscopy identified 92.4% of malaria cases, while NM-PCR detected 73.9%. The RealStar and Viasure qPCR kits detected 92.4% and 95.7% of cases, respectively. Viasure achieved the highest sensitivity (97.6%) and NPV (50%), while NM-PCR demonstrated the best specificity (71.4%). For species-level detection, RealStar was most effective in identifying Plasmodium falciparum (85%), while Viasure performed best in detecting mixed infections (34.6%). RealStar showed strong performance in detecting Plasmodium vivax (AUC = 90%). Additionally, qPCR methods were able to detect low parasitemia levels that were missed by microscopy.

Conclusions:

Among the diagnostic tools assessed, the Viasure qPCR kit showed the highest sensitivity and was particularly effective in identifying mixed infections. Although light microscopy maintained better specificity and PPV, qPCR provided a significant advantage in detecting cases with low parasitemia, suggesting its important role in enhancing the accuracy of malaria diagnosis.

InsightAce Analytic Pvt. Ltd. has published a new market evaluation report titled "One Step RT-qPCR Kits Market", which analyzes the industry based on several segments, including Reaction Volume (25, 50, 100, 200, 400, 500, 1000, and above 1000 reactions), Application (Virus Detection, Gene Expression Analysis, High-Sensitivity RNA Quantification, Genetic Profiling, Mutation Detection, Multiplexing, and Single Cell RT-PCR), Detection Method (Probe-based and Dye-based), and End-User (Hospitals, Diagnostic Labs, Reference Labs, Academic & Research Institutes, and Others). The report covers market trends, competitive landscape, and revenue forecasts through 2031.

Based on the latest findings from InsightAce Analytic, the global One Step RT-qPCR Kits Market was valued at USD 109.3 million in 2023 and is projected to reach USD 152.0 million by 2031, growing at a CAGR of 4.3% from 2024 to 2031.